Determination of Reducing Sugar Groups from Hydrolysis of Arthrospira platensis Microalgae using Microwaves
(Ekstraksi Polifenol dari Kulit Pisang Tanduk (Musa Corniculata) Menggunakan Metode Microwave Assisted Extraction (MAE) dengan Pelarut Etanol)
Embedded Files
Sholeh Ma’mun
Jurusan Teknik Kimia, Fakultas Teknologi Industri, Universitas Islam Indonesia, Jl. Kaliurang Km. 14,5 Yogyakarta 55584, Indonesia
Nur Ariffa Rochmaningsih
Jurusan Teknik Kimia, Fakultas Teknologi Industri, Universitas Islam Indonesia, Jl. Kaliurang Km. 14,5 Yogyakarta 55584, Indonesia
Riqqah Nabila
Jurusan Teknik Kimia, Fakultas Teknologi Industri, Universitas Islam Indonesia, Jl. Kaliurang Km. 14,5 Yogyakarta 55584, Indonesia
Aisyah Sumalyani
Jurusan Teknik Kimia, Fakultas Teknologi Industri, Universitas Islam Indonesia, Jl. Kaliurang Km. 14,5 Yogyakarta 55584, Indonesia
DOI: https://doi.org/10.19184/bst.v12i2.47304
ABSTRACT
The depletion of fossil fuel sources and increasing carbon dioxide (CO2) emissions have encouraged research into renewable energy sources. Bioethanol is an environmentally friendly energy source with considerable potential for reducing dependence on gasoline. Bioethanol is produced from the fermentation process of monosaccharides. The first and second generations of bioethanol are derived from food crops, agricultural waste, and plantation waste, whereas the third generation is produced from microalgae. Arthrospira platensis is a carbohydrate-rich microalgae. This study attempts to determine the content of reducing sugar groups which are monosaccharides formed from a hydrolysis with microwaves. A total of 10 g of microalgae powder was added to 100 mL of 0.3 M H2SO4 solution. The hydrolysis process was carried out in a microwave reactor at a temperature of 100°C for 90 minutes. The hydrolysate obtained was then fermented with Saccharomyces cerevisiae anaerobically in a shaking water bath. The High Performance Liquid Chromatography (HPLC) test was performed to identify reducing sugar groups in the hydrolysate, and the Gas Chromatography (GC) test was performed to determine the concentration of bioethanol produced during the fermentation process. Meanwhile, the solid content of biochar that remained after hydrolysis was analyzed using Fourier-Transform Infrared Spectoscopy (FTIR). The HPLC test findings showed that the glucose concentrations before and after fermentation were 10.52 and 1.91 g/L, respectively, indicating that 81.8% of the glucose was converted to bioethanol. Furthermore, the distillation results from the fermented hydrolysate were analyzed using GC, yielding a bioethanol content of 3.90 g/L.
Keywords: Arthrospira platensis, Bioethanol, Microalgae, Microwave, Saccharomyces cerevisiae.
Published
20-06-2024
Issue
Vol. 12 No. 2 2024: BERKALA SAINSTEK
Pages
57-63
License
Copyright (c) 2024 BERKALA SAINSTEK
Page updated
Report abuse